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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been noticed in lots of tumors, which ends up in chromosome instability, and therefore may well add to tumorigenesis. This protein was found to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a essential position in advertising and marketing mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are described. Two extra splice variants were claimed but in-depth nucleotide sequence information and facts will not be but available.
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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide generally is made up of the epitope acknowledged through the antibody.
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This guidebook outlines even more probable optimization of mobile and immunoassay parameters to ensure the best possible final results are...
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This guide outlines further more probable optimization of cellular and immunoassay parameters to make sure the very best effects are...
Antibodies certain to the blocking peptide now not bind to your epitope within the concentrate on protein. By comparing the staining with the blocked antibody compared to the antibody by yourself, one can see which staining is distinct.
Target Data Cyclin E belongs to the hugely conserved cyclin family, whose associates are characterised by a extraordinary periodicity in protein abundance through the cell cycle. Cyclins operate as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation patterns which contribute on the temporal coordination of each and every mitotic party. Cyclin E forms a complex with and functions as being a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S changeover. This protein accumulates within the G1-S section boundary and is degraded as cells development as a result of S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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